Boyd Professor and Head
Office Phone: 318-675-5750
Laboratory Phone: 318-675-5759
Office Fax: 318-675-5764
Postdoctoral Study, University of Alberta Medical Center
Ph.D., 1968, University of Mississippi Medical Center
B.S., 1962, Biology and Chemistry, Loyola University - New Orleans
Herpesvirus Gene Regulation, Persistent Infection, and Pathogenesis
Herpesvirus infection usually results in cell death and production of virus, but may result in persistent infection. Using equine herpesvirus as a model, we seek to identify which of the 80 viral genes and proteins play a role in gene regulation and in mediating herpesvirus persistent infection and the molecular mechanisms involved. Using technologies of recombinant DNA, gene cloning, in vitro translation, DNA microarrays, DNA sequencing, site-specific mutagenesis, PCR, trans-activation assays and expression vector technology, we are determining the functions of the regulatory genes of both the viral genome and the genome of defective interfering particles (DIP) that mediate persistent infection. Since the DIP genome lacks most viral genes and contains hybrid forms of regulatory genes, elucidation of the alterations in viral gene regulation may reveal the molecular events required for persistent infection. Other studies seek to elucidate the role of viral-encoded microRNAs in the viral gene program and possibly in herpesvirus latency.
Ahn, B.C., J. E. Breitenbach, S. K. Kim, and D.J. O'Callaghan. 2007. The equine herpesvirus-1 IR3 gene that lies antisense to the sole immediate-early (IE) gene is trans-activated by the IE protein, and is poorly expressed to a protein. Virology 363: 15-25.
Van de Walle, G.R. S T. Peters, B C. VanderVen, D.J. O'Callaghan, and N. Osterrieder. 2008. Equine herpesvirus 1 entry via endocytosis is facilitated by V integrins and an RSD motif in glycoprotein D. J. Virol. 82: 11859-11868.
O’Callaghan, D.J. and N. Osterrieder. 2008. Herpesviruses of horses. Encyclopedia of Virology. 3rd. B. Mahy and M. Van Regenmortel (ed.) Elsevier Publishing Co., Oxford, UK. Vol. 2. 411-420.
Ebner, P.D., S.K. Kim, and D.J. O’Callaghan. 2008. Biological and genotypic properties of defective interfering particles of equine Herpesvirus 1 that mediate persistent infection. Virology 381: 98-105.
Breitenbach, J.E., P.D. Ebner, and D.J. O’Callaghan. 2009. The IR4 auxiliary regulatory protein expands the in vitro host range of equine herpesvirus 1 and is essential for pathogenesis in the murine model. Virology 383: 188-194.
Ahn, B. C., Y. Zhang, and D. J. O’Callaghan. 2010. The equine herpesvirus-1 (EHV-1) IR3 transcript downregulates expression of the IE gene and the absence of IR3 gene expression alters EHV-1 biological properties and virulence. Virology 402: 327-337
Ahn, B.C., Y. Zhang, N. Osterrieder, and D. J. O’Callaghan. 2011. Properties of an equine herpesvirus 1 mutant devoid of the internal inverted repeat sequence of the genomic short region. Virology 410: 327-335
Charvat, R. A., J. E. Breitenbach, B-C. Ahn, Y. Zhang, and D.J. O’Callaghan. 2011. The UL4 protein of equine herpesvirus 1 is not essential for replication or pathogenesis and inhibits gene expression controlled by viral and heterologous promoters. Virology 412: 366-377.
Kim, S. K., S. Kim, G. Dai, Y. Zhang, B-C. Ahn, and D. J. O’Callaghan. 2011. Identification of functional domains of the IR2 protein of equine herpesvirus 1 required for inhibition of viral gene expression and replication. Virology 417(2):430-42
All Publications: PubMed