Office Phone: 318-675-4948
Laboratory Phone: 318-675-4976
Office Fax: 318-675-5764
Postdoctoral Study, University of North Carolina at Chapel Hill
Ph.D., Virus Immunology, 1973, University of London
B.Sc., Bacteriology, 1968, University of Liverpool
Virus cell interactions, pathogenesis of oncogenic EBV
Long standing research interests are in virus cell interactions and virus pathogenesis, particularly in the biology of Epstein-Barr virus (EBV). EBV is an oncogenic human herpesvirus that establishes persistent infections in almost 100% of the world's population. Most people are infected subclinically in childhood. However, the virus also causes infectious mononucleosis, oral hairy leukoplakia and immunoblastic lymphoma and is strongly implicated in the development of Burkitt's lymphoma, nasopharyngeal carcinoma, gastric carcinoma and Hodgkin's Disease. EBV has two major cellular targets, B lymphocytes and epithelial cells. Our long term goal is to understand how the virus enters and exits these target cells and how it spreads within and between its human hosts. Studies focus on the functions of the envelope glycoproteins critical to these processes and on the cellular proteins with which they interact.
Borza, C.M., and Hutt-Fletcher, L.M. 2002. Alternate replication in B cells and epithelial cells switches Epstein-Barr virus tropism. Nature Medicine, 8:594-599.
Chesnokova, L.S., Nishimura, S.L., and Hutt-Fletcher, L.M. 2010. Fusion of epithelial cells by Epstein-Barr virus proteins is triggered by binding of viral glycoproteins gHgL to integrins αvβ6 or αvβ8. Proc. Natl. Acad. Sci. USA, 106:20464-20469.
Chesnokova, L.S., and Hutt-Fletcher, L.M. 2011. Fusion of EBV with epithelial cells can be triggered by αvβ5 in addition to αvβ6 and αvβ8 and integrin binding triggers a conformational change in gHgL J. Virol., 85:13214-13223, 2011.
Valencia, S.M. and Hutt-Fletcher, L.M. 2012. Important but differential roles for actin in trafficking of EBV in B cells and epithelial cells. J. Virol., 86:2-10.
Jiang, R., Gu, X., Moore-Medlin, T., Nathan, C., and Hutt-Fletcher, L.M. 2012. Oral dysplasia and squamous cell carcinoma: correlation between increased expression of CD21, Epstein-Barr virus and CK19. Oral Oncology, 48:836-841.
Gianni, T. Salvioli, S., Chesnokova, L.S. Hutt-Fletcher, L.M. and Campadelli-Fiume, G. αvβ6- and αvβ8-integrins serve as interchangeable receptors for HSV gH/gL and promote endocytosis and activation of membrane fusion. PLoS Pathogens, 9(12):e1003806, 2013.
Chesnokova, L.S., Ahuja, M.K., Hutt-Fletcher, L.M. Epstein-Barr Virus Glycoproteins gB and gHgL Can Mediate Fusion and Entry in Trans; Heat Can Act as Partial Surrogate for gHgL and Trigger a Conformational Change in gB. J. Virol, 88:12293-12201, 2014.
Jiang, R., Ekshyyan, O., Moore-Medlin, T., Rong, X., Nathan, S., Gu, X., Abreo, F., Rosenthal, E.L., Shi, M., Guidry, J.T., Scott, R.S., Hutt-Fletcher, L.M. and Nathan, C.O. Association between HPV/EBV co-infection and oral carcinogenesis. J. Oral Pathol. Med, 44:28-36, 2015.
Zeng, M-S., Wang, H.B., Zhang, H., Zhang, J-P., Li, Y., Zhao, B., Feng, G-K., Du, Y., Xiong, D., Zhong, Q., Liu, W-L., Du, H., Li, M-Z., Huang, W.L., Tsao, S.W., Hutt-Fletcher, L., Zeng, Y-X., and Kieff, E. Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells. Nature Comm, Feb11;6:6240 2015.
All Publications: PubMed